UJI AKTIVITAS ANTIOKSIDAN SEDIAAN SERUM WAJAH EKSTRAK ETANOL DAUN KELOR (Moringa oleifera Lam.) DENGAN METODE DPPH

Abstract

Moringa leaves contain compounds that function as antioxidants. Moringa leaf antioxidants are classified as high, namely IC50 62.94 ppm. This study aims to see the serum formula of Moringa leaf extract has antioxidant activity by testing the DPPH method. The research method used experimental methods in the laboratory by testing the antioxidant activity of facial serum with various concentrations which was carried out by immersion in DPPH solution with a maximum absorption of 517nm. The results of the formulation of facial serum preparation of ethanol extract of Moringa leaves were homogeneous for 28 days of storage, the pH value of the preparation was 4.5-6.85, the viscosity value of the preparation was 3800-10780 cPs which decreased at all storage temperatures, the specific gravity value was 1.02-1, 04 gr/mL, spreadability value of 5.3-7 cm, and the results of the microbial contamination test with the Total Plate Count test <102 colonies/mL. IC50 value of the preparation F0 = 65.41 µg/ml strong category, F1 = 115.36 µg/ml medium category, F2 = 40.24 µg/ml very strong category and F3 = 92.37 µg/ml strong category. Based on the results it can be concluded that the preparation of facial serum meets the requirements according to  SNI  16-4399-1996 and  has antioxidant activity with the greatest antioxidant activity found in formula 2.