COMPARATIVE CHARACTERISTICS OF ANTIBACTERIAL EFFECT OF SILVER AND NANOSILVER IN VITRO

Abstract

Rationale: The problem of the resistance of microorganisms to many classes of antimicrobial agents becomes increasingly threatening. This promotes the search of new formulations for prevention and treatment of infectious inflammation. Aim: To evaluate antibacterial effects of silver nanoparticle colloid solutions on gram-negative, gram-positive and fungal microflora compared to already known formulations based on silver salts and nitrates of other metals. Materials and methods: The effects of silver nanoparticle colloid solutions (with concentration of nanoparticles of 50 and 100 mg/mL, particle diameter of 15±5 nm) on the microorganism growth were studied in Staphylococcus aureus (# 209P), Escherichia coli (# 26941), Klebsiella pneumoniae (#  43062) and clinical isolates of Candida albicans. For comparison, silver proteinate, nitrofural, and solutions of NaNO₃, Sn(NO₃)₂, Co(NO₃)₂ and Zn(NO₃)₂ at equimolar concentrations to AgNO₃ 1% were used. Results: After the plates with test cultures were treated with silver nanoparticle solutions and with comparator solutions, there was sheer culture growth in the areas of silver nanoparticle application (at both concentrations) and no growth in the areas of the comparator solutions application. Conclusion: The results obtained indicate that silver nanoparticle colloid solutions 50 and 100 mg/mL do not influence the growth of the studied cultures, whereas the comparator solutions exert an advanced antibacterial effect.