Somatic Embryogenesis and Micropropagationin Teasle Gourd

Abstract

Abstract.-In vitro somatic embryogenesis and subsequent plant regeneration was achieved in callus cultures derived from internode, node, shoot tip, petiole and leaf explant of teasle gourd plant on semi-solid Murashige and Skoog (MS) basal salts and growth regulators supplemented with 1.0mgl-1 BAP, 0.1mgl-1 NAA and 30 g l-1 (w/v) sucrose. Somatic embryos proliferated rapidly by somatic embryogenesis after 4 weeks. The embyogenic callus germinated on MS salts and growth regulators supplemented with 1.0mgl-1 BAP and 0.1mgl-1 NAA. The embryo-derived plantlets were transfered on half MS media with 0.3 mgl-1 IBA and sufficient rooting was achieved. Plantlets were acclimatized in the controlled environment