Design, construction, and two applications for an automated flow cell polarization fluorometer with digital read out: Enzyme inhibitor (antitrypsin) assay and antigen antibody (insulin insulin antiserum) assay

Abstract

The authors constructed and automated a flow cell polarization fluorometer and demonstrated two specific clinical applications of fluorescence polarization assay; the enzyme inhibitor assay of antitrypsin in serum, and the antigen antibody assay of insulin and its antibody. A signal is displayed directly and is immediately available for chart recording and (or) digital data processing. Since fluorescence polarization offers a number of choices in assay parameters and the use of different fluorescent probes, its development for rapid simultaneous measurements of multiple components in body fluids should be possible.