Comparative efficacy of anesthetics for the freshwater prawn Macrobrachium rosenbergii

Abstract

The freshwater prawn Macrobrachium rosenhergii is a commercially important culture species in the South Central United States. Two major constraints in the commercial culture of the freshwater prawn in the U.S. are poor survival during live transportation of seed-stock to growout ponds, and live transportation of pond harvested prawn to distant live markets due to the territorial and cannibalistic nature of prawn. The use of anesthetics could possibly improve transport survival; however, to date anesthetic agents have not been evaluated for use with prawn. Two trials were conducted with juvenile freshwater prawn to compare the efficacy of anesthetics commonly used on fish. The first trial was designed to identify the most promising candidates. In Study 1, tricaine methanesulfonate (MS-222),2- phenoxyethanol, quinaldine sulfate (quinaldine), clove oil, and Aqui-S™ were evaluated at 25 and 100 mg/L for 1 h in three replicate 10-L glass containers, containing five juvenile prawn each. Relative sedation level was determined every 3 min for 1 h, then recovery time and survival were measured. In Study 1, MS-222 and 2-phenoxyethanol were determined to be ineffective on prawn at all rates tested. Based on their performance in Study 1, quinaldine, clove oil, and Aqui-S™ were evaluated at 100, 200, and 300 mg/L in Study 2. Observations were determined as in Study 1. Clove oil and Aqui-S™ induced anesthesia faster and at lower concentrations than quinaldine. At the highest treatment rate (300 mg/L) prawn suffered 60% mortality in the Aqui-S™ treatment, 13% mortality in the quinaldine treatment, and 0% mortality in the clove oil treatment and control following a 1-h exposure to these concentrations. Based on these data, Aqui-S™ and clove oil applied at 100 mg/L may be suitable anesthetic treatments for prawn. Additional research is needed to determine optimal time and dose relationships to minimize stress during holding, handling, and transportation of prawn. © Copyright by the World Aquaculture Society 2005.