The effect of malt extract and incubation time on ethanol production from lignocellulose degradation of oil palm empty fruit bunches (OPEFB) using Phlebia sp. MG-60

Abstract

This study aimed to evalute the effects of the addition of malt extract and incubation time on ethanol production from lignocellulose degradation of OPEFB using Phlebia sp. MG-60. The first experiment was conducted to figure out the best treatment of the addition of malt extract (0; 0.1; 0.2; 0.3 g/L) for the degradation of lignocellulose using Phlebia sp. MG-60 for 28 days, then followed with the ethanol production which incubated into diferent time courses (0, 3, 5, and 7 days). Total reducing sugar (TRS), total soluble phenol (TSP), pH, lignin concentration, weight loss and ethanol concentration were analyzed to identify the changes on the breakdown of lignocellulose. The best treatment was obtained from the addition of malt extract 0.2 g/L with the highest amount on TRS (26.489 mg/g) incubated for 15 days. While weight loss (19.01%) and the lowest lignin residue (12.61%) were obtained from culture incubated for 28 days. Meanwhile the highest ethanol concentration was 0.28% obtained from 5 days culture.