Intracellular mRNA imaging with a hybridization sensitive fluorescent nucleotide

Abstract

We reported a technique for detecting intracellular RNA with a newly developed functional probe. To detect mRNAs, a polyA tail targeting probe was synthesized. This probe showed quite large enhancement in the presence of its complementary RNA. In living cultured cells, the probe also showed strong fluorescence. This technique was effective for intracellular mRNA detection.