Direct retransformation of yeast with plasmid DNA isolated from single yeast colonies using rolling circle amplification

Abstract

We have efficiently amplified plasmid DNA from single yeast colonies using rolling circle amplification (RCA). The amplified DNA can be directly used for restriction digestion, DNA sequencing, of yeast transformation. The RCA-based high-fidelity amplification would be useful for plasmid manipulation in a variety of yeast-based systems, particularly for high-throughput analyses.