Identification of different Listeria monocytogenes strains by surface enhanced FT Raman spectroscopy

Abstract

Recently developed genetic methods, compared to the traditional morphological and biochemical tests, have been designated as “gold standard” for bacterial identification. However, these methods are time consuming and require expensive reagents and expendables. In this context Raman spectroscopy is gaining increasing attention. Our study demonstrates FT Raman spectra of thermotolerant and thermoresistant Listeria monocytogenes species, applying silver nanoparticles for enhancement of the Raman signal. The region 600–800 cm-1 of SERS spectra of pathogenic bacteria is best suited for identification and discrimination of these two pathogenic bacteria.