In vitro response of the human breast cancer cell line MDAMB-231 and human peripheral blood mononuclear cells exposed to 60Co at single fraction

Abstract

Radiotherapy using gamma rays is a common modality of breast cancer treatment. The aim of this research is toinvestigate the biological response of the human breast cancer cell line MDAMB-231 and human peripheral bloodmononuclear cells (PBMC) exposed in vitro to 60 Co irradiation at a single fraction of 10 Gy, 25 Gy and 50 Gy doses at136,4 cGy.min -1 rate. Cells were irradiated at room temperature by the Theratron 80 radiotherapy system. Biologicalresponse was evaluated through cellular viability using MTT assay and nucleus damages visualized by PropidiumIodide assay and electrophoresis agarose gel after gamma irradiation. Nucleus damages induced by 60Co irradiationwere compared to damage caused by cell exposure to 10% methanol. The 50 Gy dose of irradiation did not stimulatenuclus damages at the same level as that affected by 10% methanol induction in the MDAMB-231. Further studies arenecessary to understand these mechanisms in the MDAMB-231 human breast carcinoma cell line.