Induction of a 5-lipoxygenase product by daidzein is involved in the regulation of influenza virus replication

Abstract

This study was conducted to evaluate the regulation mechanism of influenza virus replication following treatment of MadinnDarby canine kidney cells with the soy isoflavone daidzein. We performed comparative qualitative and quantitative analyses of lipid peroxide between mockkinfected and virussinfected cells treated with or without daidzein, as it had been reported that daidzein was an antioxidant and lipid peroxide levels increased upon virus infecc tion. Contrary to our belief, lipid peroxides were not elevated in virussinfected cells and no decrease in lipid peroxides was observed in daidzeinntreated cells. In daidzeinntreated cells, 55 hydroxyeicosatetraenoic acid, the 55lipoxygenase product derived from arachidonate, was significantly elevated compared to other lipid peroxides. Zileuton (55lipoxygenase inhibitor) and 55 lipoxygenase knockdown reduced the daidzeinninduced antiviral effect. Moreover, virus replication was regulated by treatment with 55hydroperoxyeicosatetraenoic acid, a precursor of 55 hydroxyeicosatetraenoic acid and 55lipoxygenase primary product. These results suggest that daidzein regulates virus replication via signal transduction through 55lipoxygenase products.