Isolation of phenol-degrading Bacillus stearothermophilus and partial characterization of the phenol hydroxylase

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Abstract

Bacillus stearothermophilus BR219, isolated from river sediment, degraded phenol at levels to 15 mM at a rate of 0.85 μmol/h (4 x 106 cells). The solubilized phenol hydroxylase was NADH dependent, exhibited a 55°C temperature optimum for activity, and was not inhibited by 0.5 mM phenol.

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Gurujeyalakshmi, G., & Oriel, P. (1989). Isolation of phenol-degrading Bacillus stearothermophilus and partial characterization of the phenol hydroxylase. Applied and Environmental Microbiology, 55(2), 500–502. https://doi.org/10.1128/aem.55.2.500-502.1989

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