Deep Learning–Based Segmentation of Trypanosoma cruzi Nests in Histopathological Images

Abstract

The use of artificial intelligence has shown good performance in the medical imaging area, in particular the deep learning methods based on convolutional neural networks for classification, detection, and/or segmentation tasks. The task addressed in this research work is the segmentation of amastigote nests from histological microphotographs in the study of Trypanosoma cruzi infection (Chagas disease) implementing a U-Net convolutional network architecture. For the nests’ segmentation, a U-Net architecture was trained on histological images of an acute-stage murine experimental model performing a 5-fold cross-validation, while the final tests were carried out with data unseen by the U-Net from three image groups of different experimental models. During the training stage, the obtained results showed an average accuracy of 98.19 ± 0.01, while in the case of the final tests, an average accuracy of 99.9 ± 0.1 was obtained for the control group, as well as 98.8 ± 0.9 and 99.1 ± 0.8 for two infected groups; in all cases, high sensitivity and specificity were observed in the results. We can conclude that the use of a U-Net architecture proves to be a relevant tool in supporting the diagnosis and analysis of histological images for the study of Chagas disease.