Diversity induction with gamma-ray irradiation on Phalaenopsis amboinensis var. Common natural orchid generation M1V0

Abstract

Phalaenopsis is a popular genus in the Orchidaceae family. Phalaenopsis amboinensis orchid is an endangered natural orchid, so it needs to be preserved. Conservation of natural orchid Phalaenopsis amboinensis var. Common way is by increasing genetic diversity. This research aims to determine the characteristics and morphological diversity of Phalaenopsis amboinensis natural orchid irradiated gamma-ray results and without gamma-ray irradiation; this research was conducted in two places: The implementation of gamma-ray irradiation at the PAIR-BATAN Laboratory and in Matesih, Karanganyar with an average height of 450 m above sea level. The research was conducted from December 2022 to May 2023. The study used a simple design of one factor, the doses of gamma-ray irradiation with 5 levels: 0 Gy (R0), 7.5 Gy (R1), 15 Gy (R2), 22.5 Gy (R3), 30 Gy (R4). Each treatment contained five accessions. So, there are 25 experimental units. Observational data were analyzed descriptively by comparing observational data from Phalaenopsis amboinensis var. Common Generation M1V0 orchids irradiated with control plants. The results showed diversity in the morphology of the Phalaenopsis amboinensis var. Common orchid Generation M1V0 results from gamma-ray irradiation based on qualitative morphology with phenotypic observations, showing two orchid leaf shapes: oblanceolate and elliptic. There are accessions with fasciated root conditions.