Selección de cepas de Trichoderma spp. generadoras de metabolitos secundarios de interés para su uso como promotor de crecimiento en plantas cultivadas

Abstract

The Trichoderma-Trichoderma interaction of 35 strains was studied because of the fungal potential to synthesize Secondary Metabolites (MS), such as the 3-indoleacetic acid (IAA), by using dual confrontations in solid crop of potato-dextrose-agar (PDA) as a selecting method for forming strains of "defense barrier" between 0.5-0.7cm with an optimum width selection. 20 dual confrontations were identified. These were replicated in liquid culture potato- dextrose broth (PDB) under constant stirring. After 9 days of growth, the filtration was performed to separate the metabolites synthesized by the fungus mycelium formed. This substance of interest, containing the MS, was inoculat- ed into two crops: Lactuca sativa and Raphanus sativus. It was found that the best treatments for testing are the Lactuca sativa 3(BP-T0001-BPT0029), 4(BP-T0006-BPT0028),7(BP-T0007-BPT0024) confrontations and the individual crop I (BP- T0028) as the best inducer strains of growth and involving weight, length , root volume and leaf weight . For testing of Raphanus sativus, the best treatments are the confrontation 10 (BP- T0015 - BPT0025) and the individual K crop (BP-T0031) as inducers of root length, diameter and bulb weight. The individual G crop (BP-T0024) and the chemical fertilizer QUI, inducers of leaf weight and the confrontation 3(BP- T0001-BPT0029) as inducer of root weight. Therefore, the MS synthesized by Trichoderma spp. MS during dual confrontations act as Growth