Trichoderma species Cellulases Produced by Solid State Fermentation

Abstract

The main aim of this study was to analyze eight species of Trichoderma for cellulase enzyme production by solid state fermentation. Different carbon sources such as wheat bran, corn cob, sucrose, maltose and filter paper were used. Highest celluase enzyme production was achieved with T. harzianum on media supplemented with corn cob. The optimum pH, temperature and thermal stability of isolated enzymes were also analyzed. The best pH for enzyme production was found between 4-6. The optimum temperature range for cellulase production ranged between 30-40°C. Choosing the optimum pH, temperature and best carbon source are essential for the enzyme production. Compare to other fungal genera it has been found that Trichoderma spp. have the greater potential to synthesize cellulase enzyme.