Exploration of bacterial diversity from the soil of citrus plantations applied with organic fertilizer and salicylic acid

Abstract

The abundance of bacteria in citrus plantations has not been widely studied. This study aims to explore and evaluate the abundance of soil bacteria on citrus plantations. Several treatments were given to them, organic fertilizer and plant resistance inducers. The research was carried out in July - September 2022 at the Innovation Garden and at the Laboratory of Plant Protection, Politeknik Negeri Jember. This study initiated the application of treatment on citrus plantations and isolation of bacteria using multilevel dilution technique. The design used was a randomized block design, which consisted of the application of organic fertilizer and salicylic acid. Organic fertilizer application consists of control, half dose, and recommended dose. Application of 100 ppm salicylic acid watered to the plant. There were 6 treatments, with 3 replications and 2 plants each, a total of 36 plants. The results showed that 45 various bacteria were found with different morphology with a total population 20x109 - 20x1011 CFU/gr. All treatments gave almost the same results. The application of 20 kg of organic fertilizer and 100 ppm of salicylic acid gave the highest bacterial diversity as shown by the number of different bacterial isolates. The combination of organic fertilizer and salicylic acid provides new information about the abundance and diversity of bacteria in citrus plantations.