Factors affecting the growth of virus of foot-and-mouth disease on surviving explants of bovine tongue epithelium

Abstract

A simple, laboratory scale, stirred system has been developed to examine conditions of culture necessary for optimum return of infective virus from explants of surviving bovine tongue epithelium, with particular bias towards large-scale procedures. Control of hydrogen-ion concentration and aeration of the culture are emphasized. The authors wish to thank Dr I. A. Galloway and Dr J. B. Brooksby for their helpful advice in preparing this paper, Mrs J. Lansley for her untiring technical assistance and Mr J. Goodridge who constructed the culture apparatus. © 1964, Cambridge University Press. All rights reserved.