Trafficking of Plasmodium falciparum chimeric rhoptry protein with brefeldin A

Abstract

Trafficking of the rhoptry chimeric protein RhopH2- GFP, which contains RhopH2 signal peptide plus the downstream five amino acids, was dissected by treating parasites with Brefeldin A at three different time points. Twenty eight hrsstage trophozoites accumulated the chimera within the parasite endoplasmic reticulum. In 32 hrs-stage schizonts, the chimera was distributed in the parasite cytoplasm but not in the parasitophorous vacuole. In 36 hrs stage-schizonts, the chimera was detected in individual structures around the developing merozoites and, in contrary to non-treated parasites, no immature rhoptry vesicles could be detected in the cytoplasm of immature merozoites. These data show that this chimera is trafficked to the rhoptries via Brefeldin A-sensitive pathway indicating that this trafficking is similar to that of the endogenous rhoptry proteins, and that the five amino acids downstream of the signal peptide cleavage site may contain the sorting signal required for rhoptry targeting. © Institute of Parasitology, Biology Centre ASCR.