Mutations in the PAY5 gene of the yeast Yarrowia lipolytica cause the accumulation of multiple subpopulations of peroxisomes

Abstract

We previously reported the cloning of the PAY5 gene of the yeast Yarrowia lipolytica by complementation of the peroxisome assembly mutant pay5-1 (Eitzen, G. A., Titorenko, V. I., Smith, J. J., Veenhuis, M., Szilard, R. K., and Rachubinski, R. A. (1996) J. Biol. Chem. 271, 2030020306). The peroxisomal integral membrane protein Pay5p is a homologue of mammalian PAF- 1 proteins, which are essential for peroxisome assembly and whose mutation in humans results in peroxisome biogenesis disorders. Mutations in the PAY5 gene result in the accumulation of three distinct peroxisomal subpopulations. These subpopulations are characterized by differences in 1) buoyant density, 2) the relative distribution of peroxisomal matrix and membrane proteins, 3) the efficiency of import of several peroxisomal matrix proteins, and 4) the phospholipid levels of peroxisomal membranes. These data, together with the analysis of temporal changes in the relative abundance of individual peroxisomal subpopulations in pay5 mutants, suggest that these subpopulations represent intermediates in a multistep peroxisome assembly pathway normally operating in yeast cells.