Microfluidic arrays for bioimprint of cancer cells

Abstract

We report the development and characterization of a microfluidics-based bioimprint process using high-density microchannel arrays for cell-culture and polymer delivery. The tubeless PDMS arrays consist of multiple independent microchannels and allow for parallelized bioimprint via automated dispensing and passive pumping. Using the microchannels, a 400 nm thin test pattern was replicated into a methacrylate biopolymer to demonstrate process applicability. Bioimprints of cobalt chloride stimulated Ishikawa endometrial cancer cells exhibiting exocytosis-like pore structures were compared with controls using AFM to exemplify a process application. The devices can be used for high-throughput cell assays, cell developmental studies and the formation of phenotype-specific biomimetic scaffolds. © 2011 Elsevier B.V. All rights reserved.