Establishment of an ASPL-TFE3 renal cell carcinoma cell line (S-TFE)

Abstract

Xp11 translocation renal cell carcinoma is a rare disease diagnosed in children and adolescents in the advanced stage with an aggressive clinical course. Various gene fusions including the transcription factor e3 (TFe3) gene located on chromosome X cause the tumor. We established an Xp11 translocation renal cell carcinoma cell line from a renal tumor in a 18-y-old Japanese female and named it 's-TFe.' The cell line and its xenograft demonstrated deinite gene fusion including TFe3. They showed strong nuclear staining for TFe3 in immunohistochemistry, TFe3 gene rearrangement in dual-color, break-apart FIsh analysis and aspL-TFe3 type 1 fusion transcripts detected by RT-pCR and direct DNa sequencing. although many renal cell carcinoma cell lines have been established and investigated, only a few cell lines are recognized as Xp11.2 translocation carcinoma. s-TFe will be useful to examine the characteristics and drug susceptibility of Xp11 translocation renal cell carcinoma. © 2013 Landes Bioscience.