Cell migration and apoptosis in human lung cancer cells by Clove (Syzygium aromaticum) dried flower buds extract

Abstract

In vitro inhibition of cell migration/wound closure and cellular apoptosis was studied of four chloroform extract fractions of clove (Syzygium aromaticum L.) on human A549 and H1299 cancer cell lines. The flow cytometry analysis was carried out with A549 and H1299 cancer cell lines. The results indicated that chloroform extract fractions of clove showed inhibition of wound closure/cell migration of A549 and H1299 and induced apoptosis of H1299. This was demonstrated by calculating low wound closure percentage in cells; treated with these fractions as compared to control (70%). The morphological features of the nuclei of the cells treated with fractions indicated chromatin compression, nuclear shrinkage and  apoptotic bodies formation, which pointed out towards death by apoptosis. The flow cytometer analysis of the treated cancer cells H1299 also revealed death due to the apoptosis. These results confirmed that the chloroform extract of clove bud may be used to treat lung cancer.