Construction and analysis of deletion mutations in the U5 region of Moloney murine leukemia virus: effects on RNA packaging and reverse transcription

Abstract

A collection of deletion mutations was generated in the U5 region of cloned DNA copies of Moloney murine leukemia virus or a related retrovirus. Cell lines expressing the mutant DNAs were generated by cotransformation, and the virions released were characterized biochemically. Deletions in the 5' part of U5 profoundly reduced packaging of the viral RNA into virions; one deletion in the 3' part of U5 did not block packaging but affected reverse transcription. One mutant with a deletion in the central part of U5 was fully viable and served to separate the two functional parts of U5.