TWIGS OF Andrographis paniculata (Burn. F) NEES ATTENUATES CARBON TETRACHLORIDE (CCl4-) INDUCED LIVER DAMAGE IN WISTAR ALBINO RATS

Abstract

This research's primary focus was to evaluate the hepatoprotective potentials of Andrographis paniculata in carbon tetrachloride-induced hepatotoxicity in Wistar rats. A total of thirty-six (36) Wistar rats were used for this study. The animals were shared equally into six (6) groups. Three of the six groups (1, 3, and 5) served as control and were given distilled water (1 ml/kg), Silymarin (50 mg/kg), and Andrographis paniculata (500 mg/kg) group. The administration duration was 28 days, after which they were given olive oil and normal saline (1 ml/kg). The other groups (2, 4, and 6) were treated similarly as 1, 3, and 5 for 28 days; after that, they were administered a single dose of carbon tetrachloride in olive oil on the 28th day. All animals were sacrificed on the 29th day after an overnight fast. Animal weights were assessed once a week during the research. Biochemical parameters were measured using the spectrophotometry method. Significant changes (p < 0.05) in the activities of hepatic markers (Alanine transaminase, Aspartate transaminase, Alkaline phosphatase), and concentrations of triglyceride and high-density lipoprotein in the carbon tetrachloride-induced group treated with Andrographis paniculata in comparison with group 2 (negative control) were observed. The outcomes demonstrated that the ethanolic extract of Andrographis paniculata has hepatoprotective properties against carbon tetrachloride-induced liver injury.