Assignment of randomly amplified polymorphic DNA markers to all chromosomes of shallot (Allium cepa L. Aggregatum group)

Abstract

The chromosomal locations of randomly amplified polymorphic DNA (RAPD) markers were examined in shallot (Allium cepa L. Aggregatum group). A series of alien monosomic addition lines of Japanese bunching onion (A. fistulosum L.) with extra chromosomes (1A-8A) from A. cepa Aggregatum group was used as plant materials. Several decamer (OPERON KITs A, E, and G) and 12-mer (Wako DNA Oligomer sets A-1, C-4, F-4, and F-5) oligonucleotides were applied as random primers. The chromosomal locations of the 16 RAPD markers were determined; the RAPD marker OPE091600 was located on the chromosome 1A of A. cepa Aggregatum group, OPE03600 and OPE181400 on 2A, OPA12700 on 3A, OPA11950, OPE181500, OPG10800, WAA091800, WAF65900, and WAF83800 on 4A, OPE17500 and OPE182000 on 5A, OPG08500 on 6A, WAF812800 and WAF832200 on 7A, and WAC68500 on 8A. Consequently, the RAPD markers were assigned to all the chromosomes of A. cepa Aggregatum group.