Determination of Poly-β-Hydroxybutyrate and Poly-β-Hydroxyvalerate in Activated Sludge by Gas-Liquid Chromatography

  • Comeau Y
  • Hall K
  • Oldham W
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Abstract

A convenient gas-liquid chromatography procedure to quantify poly-β-hydroxybutyrate and poly-β-hydroxyvalerate in activated sludge was developed by combining lyophilization of the samples, purification of the chloroform phase by water reextraction, and the use of capillary columns. With a flame ionization detector the sensitivity was estimated at 10 −5 g/liter.

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Comeau, Y., Hall, K. J., & Oldham, W. K. (1988). Determination of Poly-β-Hydroxybutyrate and Poly-β-Hydroxyvalerate in Activated Sludge by Gas-Liquid Chromatography. Applied and Environmental Microbiology, 54(9), 2325–2327. https://doi.org/10.1128/aem.54.9.2325-2327.1988

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