Response of the Vanda Planlet (Vanda sanderiana) to the Addition of Guano and Mycorrhizal Fertilizers in the Acclimatization Stadia

Abstract

Acclimatization is a critical point for the success of tissue culture propagation. In this phase, in addition to maintaining the condition of the growing environment, accuracy of media composition is required. This study aims to determine the growth response of the Vanda orchid plantlet (Vanda sanderiana) acclimatization phase to the dose of guano and mycorrhizal fertilizers. The design used in this study was a Factorial Randomized Block Design (RBD) with 2 factors. The first factor is the dose of guano fertilizer (without guano, 4g/plant and 8 g/plant), the second factor is mycorrhizal dose (without mycorrhizal, 10 g/plant and 20 g/plant), repeated 3 times. Growth observation variables include number of leaves, leaf length, leaf width and life percentage. The experimental results were analyzed using Analysis of Variace and followed by 1% BNJ test. The results showed that the interaction of the two treatment factors did not significantly affect all parameters. Conclusions guano and mycorrhizal fertilizers are not needed in the acclimatization phase of the Vanda orchid plantlet.