Light scattering measurements for quantifying biological cell concentration: An optimization of opto-geometric parameters

Abstract

An experimental study was carried out, aimed at optimizing the opto-geometric configuration for measuring the concentration of biological cells by means of static light scattering measurements. A LED-based optoelectronic setup making use of optical fibers was experimented, as the precursor of a low-cost device to be integrated in instrumentation for cytometry. Two biological sample types were considered as test samples of the most popular analyses - cervical cells and urine, respectively. The most suitable wavelengths and detecting angles were identified, and calibration curves were calculated.