Green synthesis of silver nanoparticles from oxynema thaianum alu pbc5 and their in vitro and in vivo activity against ESBL producing MDR Escherichia coli and klebsiella pneumoniae

Abstract

Antibiotic resistance is a huge problem that stays to challenge the healthcare sector in the world. The spread of multi drug resistant (MDR) bacteria remains a widely an unsolved problem. In the present study, antibacterial efficacy of cyanobacterium, Oxynema thaianum mediated silver nanoparticles (AgNPs) against extended-spectrum β-lactamases (ESBL) producing MDR strains were investigated. The UV-visible spectrum of biosynthesized AgNPs exhibited a characteristic SPR peak at 430 nm. The FT-IR, XRD, FESEM, EDAX and HRTEM analysis results confirms that the synthesized AgNPs were crystalline nature and spherical to oval in shape with size of 8-50 nm. The AgNPs synthesized from O. thaianum demonstrates high inhibitory activity in disc diffusion, MIC and MBC assays against MDR Escherichia coli and Klebsiella pneumoniae. Moreover, The LC50 value of AgNPs against A549 cells and Artemia nauplii were recorded as 7.2 and 53.33 μg mL-1, respectively. Therefore the study concluded that O. thaianum mediated AgNPs can be used as an alternative biological agent to control ESBL producing MDR pathogens.