Protective Effect of Kaempferol on the Transgenic Drosophila Model of Alzheimer’s Disease

Abstract

BACKGROUND: Alzheimer's disease (AD) is characterized by the accumulation and deposition of beta-amyloid peptides leading to a progressive neuronal damage and cell loss. Besides several hypotheses for explaining the neurodegenerative mechanisms, oxidative stress has been considered to be one of them. Till date, there is no cure for AD, but the pathogenesis of the disease could be delayed by the use of natural antioxidants. In this context, we decided to study the effect of kaempferol against the transgenic Drosophila expressing human Abeta-42. METHOD: The AD flies were allowed to feed on the diet having 10, 20, 30 and 40microM of kaempferol for 30 days. After 30 days of exposure, the Abeta-flies were studied for their climbing ability and Aversive Phototaxis Suppression (APS) assay. Abeta-flies' head homogenate was prepared for estimating the oxidative stress markers, Caspase, and acetyltcholinesterase activity. RESULTS: The results of the present study reveal that the exposure of AD flies to kaempferol delayed the loss of climbing ability, memory, reduced the oxidative stress and acetylcholinesterase activity. CONCLUSION: Kaempferol could be used as a possible therapeutic agent against the progression of the Alzheimer's disease.