Isolation and cloning of porcine SLC27A2 gene and detection of its polymorphism associated with growth and carcass traits

Abstract

The protein encoded by SLC27A2 gene is an isozyme of long-chain fatty-acid-coenzyme A ligase family, and it converts free long-chain fatty acids into fatty acyl-CoA esters, and thereby plays a key role in lipid biosynthesis and fatty acid degradation. In the present study, SLC27A2 located on human chromosome 15 was selected as candidate gene and we isolated and cloned partial fragments of mRNA sequence and genomic fragments of porcine SLC27A2 gene. The coding region of the gene as determined by alignments shared 90% and 82% identity with human and mouse cDNAs, respectively. Detection in Large White and Meishan breeds showed that a single nucleotide polymorphism (SNP) (A-→G) existed in exon 7, which caused corresponding amino acid changed for encoding. In LargeWhite pigs it encoded for Val while in Meishan pigs it encoded for Ilie, so we developed the PCR-RFLP genotype method for detection of this polymorphism. Association study in 135 F2 reference family indicated that significant correlation existed between the polymorphism and growth and carcass traits.