Physiological, biochemical and molecular responses to a combination of drought and ozone in Medicago truncatula

Abstract

Drought and tropospheric ozone are escalating climate change problems that can co-occur. In this study, we observed Medicago truncatula cultivar Jemalong that is sensitive to ozone and drought stress when applied singly, showed tolerance when subjected to a combined application of these stresses. Lowered stomatal conductance may be a vital tolerance mechanism to overcome combined ozone and drought. Sustained increases in both reduced ascorbate and glutathione in response to combined stress may play a role in lowering reactive oxygen species and nitric oxide toxicity. Transcriptome analysis indicated that genes associated with glucan metabolism, responses to temperature and light signalling may play a role in dampening ozone responses due to drought-induced stomatal closure during combined occurrence of these two stresses. Gene ontologies for jasmonic acid signalling and innate immunity were enriched among the 300 differentially expressed genes unique to combined stress. Differential expression of transcription factors associated with redox, defence signalling, jasmonate responses and chromatin modifications may be important for evoking novel gene networks during combined occurrence of drought and ozone. The alterations in redox milieu and distinct transcriptome changes in response to combined stress could aid in tweaking the metabolome and proteome to annul the detrimental effects of ozone and drought in Jemalong. Drought and ozone are two important aspects of global climate change that are likely to co-occur. Medicago truncatula cv. Jemalong is sensitive to drought and ozone, when the stressors are applied singly. However, Jemalong plants exhibited tolerance to combined application of drought and ozone. In this study we show that Jemalong plants perceive combined ozone and drought stress as a new 'stress state' mediated by unique changes in their redox milieu and transcriptional profiles. © 2012 Blackwell Publishing Ltd.