Antioxidant Activity of the Ethanol Extract of Manilkara zapota Leaf

Abstract

The present study evaluated the antioxidant activity of cold ethanolic extract of Manilkara zapota (Sapotaceae) leaves. In vitro antioxidant activity was determined using 1, 1-diphenyl-2-picrylhydrazyl radical, reducing power capacity, total phenol and flavonoid content. The extract demonstrated significant dose dependent antioxidant activity in vitro methods. In DPPH radical scavenging assay IC50 values of Manilkara zapota leaves (MZL) and ascorbic acid (standard) were found to be 68.27 and 16.17 μg/ml, respectively. In vivo, the extract was evaluated by carbon tetrachloride (CCl4) induced liver damage rats in hepatoprotective model. CCl4 produced significant alteration of serum marker enzymes, total bilirubin, total protein and liver weight. Restoration of these values towards normal, which is comparable to control group, indicated hepatoprotective activity, which reflects the antioxidant potential of the extract. Results presented here indicate that MZL possess strong antioxidant activity and they can therefore be used as a good natural source of antioxidant.Keywords: MZL; DPPH; Scavenging activity; Serum marker enzymes.© 2012 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.doi: http://dx.doi.org/10.3329/jsr.v4i1.7148J. Sci. Res. 4 (1), 193-202 (2012)