Isolation and identification of microfungi from soils in serdang, selangor, malaysia

Abstract

Microfungi are commonly inhabited soil with various roles. The present study was conducted in order to isolate and identify microfungi from soil samples in Serdang, Selangor, Malaysia. In this study, the soil microfungi were isolated using serial dilution technique and spread plate method. A total of 25 isolates were identified into ten genera based on internal transcribed spacer region (ITS) sequence analysis, namely Aspergillus, Clonostachys, Colletotrichum, Curvularia, Gliocladiopsis, Metarhizium, Myrmecridium, Penicillium, Scedosporium and Trichoderma consisting 18 fungi species. Aspergillus and Penicillium species were claimed as predominant microfungi inhabiting the soil. Findings from this study can be used as a checklist for future studies related to fungi distribution in tropical lands. For improving further study, factors including the physicochemical properties of soil and anthropogenic activities in the sampling area should be included.