Fluorescence Spectra Measurement of Essential Oils

Abstract

Identifying materials using fluorescence spectroscopy methods have several advantages including fast, accurate, and relatively low cost. This method is used not only to identify the material but also to detect whether the material is still pure or not. The purity of patchouli oil has been identified by observing and analyzing the fluorescence spectrum (λabs = 509 nm, λeks = 542nm). The observations were made for pure and mixed (impure) patchouli oil, respectively. By comparing the two data, the patchouli oil is distinguished and the contained mixture is determined. The measurement results show that the mixture oil spectrum is indicated by a shift in the maximum wavelength (λem, max) of fluorescence emissions. Nevertheless, the intensity of the fluorescence beam is change.