Stomatal density, chlorophyll content and phylogenetic relationship in 17 cultivars of Musa spp.

Abstract

The objective of the work was to study stomatal characters, chlorophyll and their phylogenetic relationship in 17 cultivars of Musa spp. (AAB; ABB; AAA; AA), of the Germplasm Bank of la Universidad Técnica Estatal de Quevedo. The experiments were carried out in the Laboratory of Microbiology and Molecular Biology. Two methods of morphometric and physiological leaf evaluations are used: a) From leaf tissue of 25 cm2 the abaxial and adaxial stomatal density was evaluated in (e/mm2) and b) From leaf disks of 1,3 cm2, was determined the total chlorophyll content. In molecular characterization by RAPD, were used five oligonucleotides. Significant statistical differences were determined (p ≤ 0,05) for the variables, with results of higher abaxial stomatal density in Barraganete of 233 e/mm2 and its directly proportional relationship between stomatal density and length, where the greater the density, the shorter its length. The chlorophyll content of 4,61 mg/g in Guineo seda. The phylogenetic tree generated two large groups at its genomic accession. Sub-group A brought together nine accessions to the genome (AAB), Sub-group B with five accessions of (AAA), highlighting Subgroup C containing the cultivar Orito of the genotype (AA) and Sub-group D with the accessions (AAB and ABB).