Effect of salt stress in the selection of salt tolerant hybrids in rice (Oryza sativa L.) under in vitro and in vivo condition

Abstract

In the present study, the salt tolerant genotypes were selected in both in vitro and in vivo condition. For in vivo condition all the genotypes were raised in three different salt affected areas having different soil and water pH and EC and observations were made on leaf proline content, Na+:K + ratio, chlorophyll stability index and single plant yield. The following hybrids TS 6/TRY 1, TS 6/BTS 24, TS 29/CSR 27, TS 29/BTS 24, IR 58025 A/TRY 1, IR 58025 A/BTS 24 were stable for most of the traits in all the environments. Under in vitro condition, the response of plant cell to salt stress and the feasibility of selecting salt tolerant callus were studied. Callus was grown on agar solidified media containing 0.2, 0.4, 0.6, 0.8 and 1.0% NaCl salt. Parameters such as fresh weight of callus, callus morphology and proline content were studied. The callus growth decreased with increasing NaCl concentration in the medium. TS6/BTS 24, TS 6/Vytilla 1, TS 6/TRY 1, TS 29/BTS 24 and IR 58025 A/Vytilla 1 were the hybrids with high tolerance to salt stress in vitro. © 2007 Asian Network for Scientific Information.