Fluorescence Quantum Yields of Laser Dyes Determined by On-Line Computer Evaluation

Abstract

The determination of relative fluorescence quantum yields has been improved to better than 3% precision by use of a multichannel analyzer and an on-line desk-top Computer. The new combined device for data acquisition and handling as well as a new correction procedure are described. Drifts of excitation source and dark current can be omitted. The yields for laser dyes, emitting at short wavelengths, are presented in dependence on excitation wavelength. These results simplify the optimal choice of the pump conditions of laser dyes, which usually are not photostable.© 1982, Verlag der Zeitschrift. Alle Rechte vorbehalten. © 1982, Walter de Gruyter. All rights reserved.