Test characteristics of acridine orange, gram, and May-Grunwald-Giemsa stains for enumeration of intracellular organisms in bronchoalveolar lavage fluid

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Abstract

For enumeration of intracellular organisms (ICO) in bronchoalveolar lavage fluid samples, the May-Grunwald-Giemsa (MGG) stain displayed higher interobserver agreement than the acridine orange and Gram stains. The MGG stain offered a reliable enumeration of ICO when 200 cells were counted by one observer.

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De Brauwer, E., Jacobs, J., Nieman, F., Bruggeman, C., & Drent, M. (1999). Test characteristics of acridine orange, gram, and May-Grunwald-Giemsa stains for enumeration of intracellular organisms in bronchoalveolar lavage fluid. Journal of Clinical Microbiology, 37(2), 427–429. https://doi.org/10.1128/jcm.37.2.427-429.1999

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