A chromosome phylogeny of the droseraceae by using CMA-DAPI fluorescent banding

Abstract

Aldrovanda vesiculosa L., Dionaea muscipula Ellis, 20 species of Drosera L. and Drosophyllum lusitanicum Link. were investigated for a metaphase chromosome analysis by using the sequentially fluorescent CMA and DAPI staining methods. Aldrovanda vesiculosa and the Drosera species showed numerous small-sized chromosomes and some middle-sized chromosomes with no primary constriction. Dionaea muscipula had the middle-sized chromosomes with the localized centromeres and CMA-negative and DAPI-positive pericentric bands. Drosophyllum lusitanicum displayed quite large-sized chromosomes with well-defined localized-centromeres. Computer-aided measurements of metaphase chromosomes stained with CMA and DAPI showed that Aldrovanda vesiculosa and the Drosera species displayed the common features such as localization of CMA-positive and DAPI-negative satellites and non-staining region.