Saccharomyces Cerevisiae Immobilized in Alginate for Continuous Fermentation

Abstract

Saccharomyces cerevisiae BY4742 cells were immobilized in alginate and applied for continuous fermentation. Immobilization of yeast cell showed technical and economic advantages over free cell system. In the fermentation, the initial glucose concentration was 110 g/L. The hydraulic retention time (HRT) of fermentation broth was set to 24 h with a re-activate time of 12 h after first incubation period 72 h. Ethanol yield and glucose utilizing ratio were analyzed for verifying the performance of continuous fermentation and the improvement of immobilized yeasts over free yeast cells for fermentation. Comparing dates in two experimental groups, the ethanol yield could reach to about 80% and the glucose utilizing ratio had the similar pattern with averagely 50%. The ethanol yield and glucose utilizing ratio had a significant improvement with the pre-activation and the re-activation. Index