Expanded RNA-binding activities of mammalian Argonaute 2

105Citations
Citations of this article
155Readers
Mendeley users who have this article in their library.

Abstract

Mammalian Argonaute 2 (Ago2) protein associates with microRNAs (miRNAs) or small interfering RNAs (siRNAs) forming RNA-induced silencing complexes (RISCs/miRNPs). In the present work, we characterize the RNA-binding and nucleolytic activity of recombinant mouse Ago2. Our studies show that recombinant mouse Ago2 binds efficiently to miRNAs forming active RISC. Surprisingly, we find that recombinant mouse Ago2 forms active RISC using pre-miRNAs or long unstructured single stranded RNAs as guides. Furthermore, we demonstrate that, in vivo, endogenous human Ago2 binds directly to pre-miRNAs independently of Dicer, and that Ago2:pre-miRNA complexes are found both in the cytoplasm and in the nucleus of human cells. © The Author(s) 2009. Published by Oxford University Press.

Cite

CITATION STYLE

APA

Tan, G. S., Garchow, B. G., Liu, X., Yeung, J., Morris IV, J. P., Cuellar, T. L., … Kiriakidou, M. (2009). Expanded RNA-binding activities of mammalian Argonaute 2. Nucleic Acids Research, 37(22), 7533–7545. https://doi.org/10.1093/nar/gkp812

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free