Evidence of an Enzymatic Degradation of Insulin in Blood in vitro

Abstract

Haemolyzed red cells from human and rat blood possess the ability to split enzymastically the insulin molecule into its A and B chain without further degradation. The splitting of insulin incubated with different blood preparations, has been studied as a function of the degree of haemolysis, by the use of a trichloroacetic acid precipitation technique using 125I‐labelled insulin. Kinetic data are obtained using haemolysate as crude enzyme. Copyright © 1971, Wiley Blackwell. All rights reserved