Comparative Study between Avian Cell and Mammalian Cell in Production of Influenza Vaccine Shariah Compliance

Abstract

Nowadays, the manufacturing industry of the influenza vaccine always make the improvement for their production. Most of them are looking for the new production cell line which are easily scalable, high yield production of viral and highly tolerant to multiple viruses. This situations happens as the high demand of the vaccine for every year from around the world. It can be seen that the capacity for the vaccines' production are really critical in the pharmaceutical industry. However, certain Muslims' community do not gain a trust from the vaccine's ingredient itself. This is due to the production process involves blood consist of serum and cell components that may come from the prohibited animals such as pig and dog. Therefore, shariah compliance vaccine has been studied in this research. The avian cell lines are now competing with the mammalian cell currently at advance stage of commercial development for the manufacture of influenza vaccines. Avian cell line chosen are AGE1.CR.pIX cells and DuckCelt-T17 will be compared with the mammalian cells which is Vero and Madin Darby Canine Kidney (MDCK) cells in producing the shariah compliance vaccine. Different type of mediums also will be considered to produce shariah compliance vaccine. The artificial media will be compared with the natural media that contain the non-shariah components. Hence, the shariah status of the enzyme and media will be known in this research. In this study, the production of shariah compliance vaccine mainly focused on the bioreactor and simulated by using a software called SuperPro® Designer. Results gained from the simulation shows the highest yield at 72 hours which is 93.51 % conversion. These study will acknowledge the halal's status of the vaccine and contribute the development of the halal vaccine processing industry.