Effect of Pakkat (Calamus caesius blume) Ethanol Extract on Testis Tissues Histology of Diabetic Rats

Abstract

Background: Diabetes Mellitus (DM) is a metabolic disease characterized by elevated blood sugar levels or chronic hyperglycemia. Diabetes has been shown to have an adverse effect on male and female reproductive function and its impact can be seen in the increasing prevalence of infertility. Pakkat is believed to be used as a medicine for diabetes mellitus. The chemical compounds contained in pakkat consist of flavonoids, alkaloids, terpenoids/steroids, saponins, tannins and glycosides which can be useful for lowering blood sugar levels. Aim: To investigate the effectiveness of pakkat extract on Sperm Count and Motility Sperm of Male Wistar Rats with Diabetes Mellitus. Methods: This study used a laboratory experimental design (true experiment) with a post test control group design only approach. The samples in this study were 25 diabetic rats that is grouped in 5 groups: control (-) group (NaCMC), control (+) group (metformin), Treatment Group I (extract dose of 125 mg/kgBW), Treatment Group II (extract dose of 250 mg/kgBW), Treatment Group III (extract at a dose of 500 mg/kgBW) given for 2 weeks. Results: The results obtained in this research show that extract of pakkat at doses of 125mg/kgBB and 250mg/kgBB after 2 weeks could increase the number and moltility of spermatozoa. Conclusion: Overall, it can be concluded of pakkat extract could increase the number and motility of spermatozoa of Male Wistar Rats with Diabetes Mellitus.