Lysozyme-induced transcriptional regulation of tnf-α pathway genes in cells of the monocyte lineage

21Citations
Citations of this article
42Readers
Mendeley users who have this article in their library.

Abstract

Lysozyme is one of the most important anti-bacterial effectors in the innate immune system of animals. Besides its direct antibacterial enzymatic activity, lysozyme displays other biological properties, pointing toward a significant anti-inflammatory effect, many aspects of which are still elusive. Here we investigate the perturbation of gene expression profiles induced by lysozyme in a monocyte cell line in vitro considering a perspective as broad as the whole transcriptome profiling. The results of the RNA-seq experiment show that lysozyme induces transcriptional modulation of the TNF-α/IL-1β pathway genes in U937 monocytes. The analysis of transcriptomic profiles with IPA® identified a simple but robust molecular network of genes, in which the regulation trends are fully consistent with the anti-inflammatory activity of lysozyme. This study provides the first evidence in support of the anti-inflammatory action of lysozyme on the basis of transcriptomic regulation data resulting from the broad perspective of a whole-transcriptome profiling. Such important effects can be achieved with the supplementation of relatively low concentrations of lysozyme, for a short time of exposure. These new insights allow the potential of lysozyme in pharmacological applications to be better exploited.

Cite

CITATION STYLE

APA

Bergamo, A., Gerdol, M., Pallavicini, A., Greco, S., Schepens, I., Hamelin, R., … Sava, G. (2019). Lysozyme-induced transcriptional regulation of tnf-α pathway genes in cells of the monocyte lineage. International Journal of Molecular Sciences, 20(21). https://doi.org/10.3390/ijms20215502

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free