The quality of sexed sperm separated using bovine serum albumin column and extended using tris aminomethane at different temperatures

Abstract

Sexing sperms is one of the reproductive technologies that is considered as a promising alternative in the effort of reproductive efficiency to produce offspring according to the desired sex. The method for maintaining and extending the sperms can be done by using bovine serum albumin (BSA) column with Tris Buffer at room temperature and waterbath without damaging the quality of the sperms. The purpose of this study was to determine the effect of BSA - Tris Buffer extender on the quality of sexed sperms after thawing at 27C and 37C. The study was divided into two treatments; P1 was Tris extender in the temperature of 27C and P2 was Tris extender in the temperature of 37C with 6 replications. Those treatments were compared using a Paired Sample test (T-Test). The parameters observed in the present study were motility, viability, abnormalities, and intact plasma membrane. The results showed that motility, viability, abnormalities, and intact plasma membrane of the sperms X and Y at 27C and 37C after sexing did not differed significantly (P>0.05). Likewise, after equilibration, the motility of sperms X at 27C and 37C did not differed significantly (P>0.05), however, sperm Y at 27C and 37C showed a significant difference (P<0,05). Viability and abnormalities of sperms X at 27C and 37C differed significantly (P<0,05) but sperms Y at 27C and 37C did not show any significant differences. After thawing process, motility, viability, abnormalities and intact plasma membrane of the sperms X and Y at 27C and 37C did not show any significant differences. However, it can be conluded that the quality of sexed sperms in the present study is still good because it has motility above 40% and abnormalities below 20%.