Use of microsatellite markers to detect heterozygosity in an F2 generaon of a black rice and white rice cross

Abstract

This research aimed to idenfy the heterozygosity of an F2 populaon of a black rice and white rice cross using microsatellite markers. Rice was grown and harvested in a field approximately 500 m2 in size in Pakem (Sleman, Yogyakarta, Indonesia), and analyzed in the Genecs and Plant Breeding Laboratory of the Faculty of Agriculture, Universitas Gadjah Mada. The research materials consisted of an F2 S × G populaon composed of a cross of black rice (S) and white rice Situbagendit (G), female parent of black rice (S), male parent of white rice (G), chemical and organic ferlizer, chemicals and tools for molecular acvity and three microsatellite markers related to color properes (RM 220, RM 224, and RM 252). All of the plant populaons (generaon F2, parent female, parent male) were planted in the field unl their harvest. Young leaves (30 days in age aer planng) were molecularly analyzed using three microsatellite markers (RM 220, RM 224, and RM 252). This encompassed DNA isolaon, PCR reacon, and the visualizaon of the PCR results using Metaphore Agarose Gel Electrophoresis. The results showed that the percentage of the number of individual plants showing a heterozygous paern in the F2 S × G populaon was 50% (RM 220), 40% (RM 224), and 60% (RM 252). As such, the RM 252 microsatellite marker can be used for marker-assisted selecon in the crossbreeding of black rice.