Stabilities of lyophilized Staphylococcus aureus typing bacteriophages

Abstract

Staphylococcus aureus bacteriophages (25 phages), were lyophilized in aliquots 12 to 18 years ago and stored in vacuo at -20°C. Eight viruses each lost one log titer, while seventeen retained the original titers. The use of lyophilized phages provided more reproducible phage typing and reduced by 75% the complexity and cost. This important test is thus made feasible for more laboratories.